Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232827_un76 TAAACCACGACTGTGATTAATTCTATGTTGTG(+ACTCAC)GATCCCAAGGAACAATTATGAAGGAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240604232826_fmvf AGTGAGGGCTTTGTCCCAAGAAGGCTGTGTGG(+ACTCAC)GTACCAGATTCTGCAAGTGTTGAAGTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240604232824_yghj GAACTATAGAACTTCAACACTTGCAGAATCTG(+ACTCAC)GTACCCACACAGCCTTCTTGGGACAAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604232823_rrqy AGGTGGCCATGAGCACCTAATGTGGGTGCTGG(+ACTCAC)GATCCTGAGTCAATGCCTCTGCAAGAGCCGT NGG 10 8 17 8 24 0 100 5 10
    240604232821_hep2 GTGCGACGGCTCTTGCAGAGGCATTGACTCAG(+ACTCAC)GATCCCAGCACCCACATTAGGTGCTCATGGC NGG 10 8 17 8 24 0 100 5 10
    240604232820_zgkc TCCCTTCCTGGATCACAATGTCTGGGGATTAG(+ACTCAC)GATCCATCCATCATCCTAGCCAAGAACTGCA NGG 10 8 17 8 24 0 100 5 10
    240604232818_xb6v CTTCATGCAGTTCTTGGCTAGGATGATGGATG(+ACTCAC)GATCCTAATCCCCAGACATTGTGATCCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604232816_ez3d ACATTTGGAGCTCCCAATGCCTGTGTCGTTGG(+ACTCAC)GATCCAGTCTGGGAACTAGGACACATTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604232815_0it2 TGTTTGCCAAATGTGTCCTAGTTCCCAGACTG(+ACTCAC)GATCCCAACGACACAGGCATTGGGAGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240604232813_6zby CTTTAAGGTACAAGTGTAAAAACAAGTTCCGG(+ACTCAC)GATCCAGTTTCTATCCGTGTGTGTTATGGTC NGG 10 8 17 8 24 0 100 5 10