Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232757_uga5 ATGAAAAGAAGTGAGGCATTGTGGGACTCTAG(+ACTCAC)GTACCCACAGTTTTGAGACTTCTGTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604232756_49oy CACAGCTCCTTAGTTGCTGGGCAGGGCTCAAG(+ACTCAC)GATCCTTCCCCACTTCATGTGGGATGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604232754_nsp3 GTCTGGAACTCATCCCACATGAAGTGGGGAAG(+ACTCAC)GATCCTTGAGCCCTGCCCAGCAACTAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240604232753_xu7t CTGAACATTTAGAACTCAGAGCCCCATTCATG(+ACTCAC)GTACCTACAGCCCATCTGGAAAATTCTTCAA NGG 10 8 17 8 24 0 100 5 10
    240604232752_4ep7 TACTATTGAAGAATTTTCCAGATGGGCTGTAG(+ACTCAC)GTACCATGAATGGGGCTCTGAGTTCTAAATG NGG 10 8 17 8 24 0 100 5 10
    240604232750_cd8a AGGCACCTTCCTGTAGAAGAGCCCATAGTCAG(+ACTCAC)GTACCCAATTACACCTTGGTGTTGTCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240604232748_lr5u ATGCATCTTGGACAACACCAAGGTGTAATTGG(+ACTCAC)GTACCTGACTATGGGCTCTTCTACAGGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604232746_blhp GTGAGTCTTGTTCATTGTGTAGAGAAACCCAG(+ACTCAC)GTACCCAAAGTCGGCAGTCTTTCCCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240604232744_j0nh ACATGGACCCTGGGAAAGACTGCCGACTTTGG(+ACTCAC)GTACCTGGGTTTCTCTACACAATGAACAAGA NGG 10 8 17 8 24 0 100 5 10
    240604232742_33sy ACTGTGGGGCAAGGAAAGTGACACTGACCTTG(+ACTCAC)GATCCACACTGCCTGGGGTTGTGTTAGTTAG NGG 10 8 17 8 24 0 100 5 10