Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232741_5vb2 GTCAGCTAACTAACACAACCCCAGGCAGTGTG(+ACTCAC)GATCCAAGGTCAGTGTCACTTTCCTTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604232740_3t35 AAGAAGGGAGATAGACCTCAAACTAGTTCTTG(+ACTCAC)GTACCTCACCTTCATCCCCCACCCACCCACT NGG 10 8 17 8 24 0 100 5 10
    240604232739_sjy0 TCGGGAGTGGGTGGGTGGGGGATGAAGGTGAG(+ACTCAC)GTACCAAGAACTAGTTTGAGGTCTATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604232737_3wwk GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+ACTCAC)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604232735_1061 GGATGGACCATCTAGTGATTGCCATATGCAGG(+ACTCAC)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240604232734_fh22 TTAGTCATCATGGATTGTCACTCCTTGAGAAG(+ACTCAC)GATCCAGAGGTGTAGATTTGTTGGGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240604232732_pl8i GACATACTTCCCCCAACAAATCTACACCTCTG(+ACTCAC)GATCCTTCTCAAGGAGTGACAATCCATGATG NGG 10 8 17 8 24 0 100 5 10
    240604232731_h0ix GTGGGATTCTCTCTATGTGTGTCTTCTACATG(+ACTCAC)GATCCAGAGAGCAGTTCCTAATGGAAACGAT NGG 10 8 17 8 24 0 100 5 10
    240604232729_2fju GATACATCGTTTCCATTAGGAACTGCTCTCTG(+ACTCAC)GATCCATGTAGAAGACACACATAGAGAGAAT NGG 10 8 17 8 24 0 100 5 10
    240604232728_ukw3 GTTGCCCTTTGATGCTGAGATCTTCCGGTATG(+ACTCAC)GATCCTCCGTGAAGGCAGGCTCAGGGTGAGA NGG 10 8 17 8 24 0 100 5 10