Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232615_1axh TATTTACAAACGAGCACACCTCTCCATTTAAG(+ACTCAC)GATCCAGTTTGCAAGTGTACAGGAGGTTGTC NGG 10 8 17 8 24 0 100 5 10
    240604232614_flf9 TGTGGGACAACCTCCTGTACACTTGCAAACTG(+ACTCAC)GATCCTTAAATGGAGAGGTGTGCTCGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240604232613_u0xb ATTGAGGATCATTTCTAGGACAAATAAATGAG(+ACTCAC)GTACCCGCTGTAGCACAGCCCGAACCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604232611_kyr5 ACAGTCAAGGGGTTCGGGCTGTGCTACAGCGG(+ACTCAC)GTACCTCATTTATTTGTCCTAGAAATGATCC NGG 10 8 17 8 24 0 100 5 10
    240604232610_y4qu TAGTACTTATAGTGTATAAAGAATGAGAGCTG(+ACTCAC)GATCCACACATGGTTCTGGAAACTGGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604232608_omxl CATTAACCTCCCAGTTTCCAGAACCATGTGTG(+ACTCAC)GATCCAGCTCTCATTCTTTATACACTATAAG NGG 10 8 17 8 24 0 100 5 10
    240604232606_ve21 TAAATACCCGATACACAGGATATCTAATATGG(+ACTCAC)GATCCCTGTGAAAGGGGTCATGACCCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240604232605_gw6h TCAACCTGGGGGGTCATGACCCCTTTCACAGG(+ACTCAC)GATCCCATATTAGATATCCTGTGTATCGGGT NGG 10 8 17 8 24 0 100 5 10
    240604232603_tfs0 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+ACTCAC)GATCCCTGGATATGGCGGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604232600_odjz GGATGGACCATGTAGAGACCGCCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10