Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604232600_odjz GGATGGACCATGTAGAGACCGCCATATCCAGG(+ACTCAC)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240604232559_wbvj TCCTTGTAAGAAATGTTCATTGCTTGAGAAAG(+ACTCAC)GATCCTCCTTTGGTTGGTGAGCAGTTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240604232557_xlto TGTGGTAGAGAACTGCTCACCAACCAAAGGAG(+ACTCAC)GATCCTTTCTCAAGCAATGAACATTTCTTAC NGG 10 8 17 8 24 0 100 5 10
    240604232556_b4dm GCTGAATTCCGTGTGAGAACCACTCCCTTTTG(+ACTCAC)GTACCCTTCAGAGATGGAGAGCTTACGGTTT NGG 10 8 17 8 24 0 100 5 10
    240604232554_zg73 CCCTCAAACCGTAAGCTCTCCATCTCTGAAGG(+ACTCAC)GTACCAAAAGGGAGTGGTTCTCACACGGAAT NGG 10 8 17 8 24 0 100 5 10
    240604232553_r5c5 ACTCTGATGGATGCCCTGGGTTGCCTTGGTGG(+ACTCAC)GTACCTCCCAGAGACAACAAAACCTTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240604232551_tzlq AGAAAATCCTAAGGTTTTGTTGTCTCTGGGAG(+ACTCAC)GTACCCACCAAGGCAACCCAGGGCATCCATC NGG 10 8 17 8 24 0 100 5 10
    240604232549_3qk4 ATTTTTTTAAAACAGAAGTGAAAAAATGACAG(+ACTCAC)GTACCAATATTACCGTGTTGGATAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240604232548_10ia ATAAAAGAAAATTATCCAACACGGTAATATTG(+ACTCAC)GTACCTGTCATTTTTTCACTTCTGTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240604232546_lcru CAAGCCCTGACCTGGAGTCCTGGTTCATCCTG(+ACTCAC)GTACCACCCACAATGAAGACAGATCTGCTCG NGG 10 8 17 8 24 0 100 5 10