Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231648_phfn CTCTGCCTTCACCTGCAGACACCCTCCCTCCG(+ACTCAC)GTACCCTGGGCTTCTACCCTCCCTAGAATTC NGG 10 8 17 8 24 0 100 5 10
    240604231646_1ypr GATGGGAATTCTAGGGAGGGTAGAAGCCCAGG(+ACTCAC)GTACCGGAGGGAGGGTGTCTGCAGGTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604231643_2gfl CCCGCGCTGGACAGCCCAGCGCCCAATCCGGG(+ACTCAC)GATCCAGAGGGTAGGCCTGCCCTTGCACTTA NGG 10 8 17 8 24 0 100 5 10
    240604231641_trb2 TGGTTTAAGTGCAAGGGCAGGCCTACCCTCTG(+ACTCAC)GATCCCCGGATTGGGCGCTGGGCTGTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240604231640_qn6o GTTAACTGTTACAGAGGAACTGGCTTTCTGAG(+ACTCAC)GTACCTGGCATCTTGGGACGAGTGAAATCTA NGG 10 8 17 8 24 0 100 5 10
    240604231638_2vhs CTGACTAGATTTCACTCGTCCCAAGATGCCAG(+ACTCAC)GTACCTCAGAAAGCCAGTTCCTCTGTAACAG NGG 10 8 17 8 24 0 100 5 10
    240604231636_rtkd GGATCTAGCTGGCCCCTTCCCTCCCCAGCCTG(+ACTCAC)GTACCCCAGGTCCAATGACATCAGTCTGACT NGG 10 8 17 8 24 0 100 5 10
    240604231633_7e4b AAAGGAGTCAGACTGATGTCATTGGACCTGGG(+ACTCAC)GTACCAGGCTGGGGAGGGAAGGGGCCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240604231632_gv5d ACTGAGAAGAAAAGGTTTTAATAAAGAACCAG(+ACTCAC)GATCCTCCCTTCCCCGAGATGGTGGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240604231630_cqxp CCAGAACACTGCCACCATCTCGGGGAAGGGAG(+ACTCAC)GATCCTGGTTCTTTATTAAAACCTTTTCTTC NGG 10 8 17 8 24 0 100 5 10