Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231609_bkqg GCCCCAGTCACTTCCCACTCCCTGCTCTCCAG(+ACTCAC)GTACCACCCGGGTCAGTGAGATACTTGGCAT NGG 10 8 17 8 24 0 100 5 10
    240604231607_yfjr AGACCATGCCAAGTATCTCACTGACCCGGGTG(+ACTCAC)GTACCTGGAGAGCAGGGAGTGGGAAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240604231604_nmy2 TCTCACAACCACCTACAACTCCAATTCCAAGG(+ACTCAC)GATCCAATGGCATCTTCTGGCTTCTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604231602_kklp CCTGGTCCCTCAGAAGCCAGAAGATGCCATTG(+ACTCAC)GATCCCTTGGAATTGGAGTTGTAGGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240604231601_3t1w TAGTCCCAGGATTGGGGAGGTAGAGACAGGAG(+ACTCAC)GATCCAGAGTTCATGGTCCTCAGCTACATAG NGG 10 8 17 8 24 0 100 5 10
    240604231600_st6r GTTTCCTATGTAGCTGAGGACCATGAACTCTG(+ACTCAC)GATCCTCCTGTCTCTACCTCCCCAATCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604231558_pk5s TACTGGCCTGAGGTGACAGGGTGGGAGAGCTG(+ACTCAC)GATCCATGAGGGAGCATGGTAGCCCGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240604231557_51tz AGGTGGCTTTCGGGCTACCATGCTCCCTCATG(+ACTCAC)GATCCAGCTCTCCCACCCTGTCACCTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604231556_pexy CTCTCTCCAGTTAGTACCTATCTGGGATATTG(+ACTCAC)GTACCTTTGGATATGAATGTCCTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604231554_fz20 ATATTTTCTTATAAGGACATTCATATCCAAAG(+ACTCAC)GTACCAATATCCCAGATAGGTACTAACTGGA NGG 10 8 17 8 24 0 100 5 10