Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231415_us5w ATGCCCTCTTTCATGGAGCTGTTCGCACTGCG(+ACTCAC)GATCCGATTCCTCGGGCTTGGATTCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240604231414_q2kp TCTCGGGAGTGGAATCCAAGCCCGAGGAATCG(+ACTCAC)GATCCGCAGTGCGAACAGCTCCATGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240604231412_j02b TCCCTGGCCTGGGCGCCCCTTGCTGTTGGGAG(+ACTCAC)GATCCCATCCTCGAGTGCCTCCTATCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604231410_dbuq AAGGCAGGAGGATAGGAGGCACTCGAGGATGG(+ACTCAC)GATCCTCCCAACAGCAAGGGGCGCCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240604231408_g3o3 TGACTTCTGAAACGAAGAGAAAAGCACCTATG(+ACTCAC)GATCCCACTCCTTCCCATCGCTTACCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240604231406_jv65 CTGGGGAACAGGTAAGCGATGGGAAGGAGTGG(+ACTCAC)GATCCATAGGTGCTTTTCTCTTCGTTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604231405_w99v TAACCCATGAACTAGCTGTAACATAGTTTGTG(+ACTCAC)GATCCATGCACTGATGCTGTGAAGCCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240604231404_4fc4 CACAGGGGCAGGCTTCACAGCATCAGTGCATG(+ACTCAC)GATCCACAAACTATGTTACAGCTAGTTCATG NGG 10 8 17 8 24 0 100 5 10
    240604231402_ari4 CAAGCTGTCGAGAAATGGTTCTCTTTACAGTG(+ACTCAC)GATCCAGTCAGGATTGGCAGCAAGCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240604231401_bgza CAGGCAAAGGTGCTTGCTGCCAATCCTGACTG(+ACTCAC)GATCCACTGTAAAGAGAACCATTTCTCGACA NGG 10 8 17 8 24 0 100 5 10