Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231311_9bmk GATTTTGGCCTTTTTCAGTTGGGTACTATATG(+ACTCAC)GTACCAGGATGAATACATGGCCTTTAATGTT NGG 10 8 17 8 24 0 100 5 10
    240604231309_juz5 GAAGGAACATTAAAGGCCATGTATTCATCCTG(+ACTCAC)GTACCATATAGTACCCAACTGAAAAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240604231308_19d1 GCTTGCTTCATAAGCATGAAGACTGCCATTTG(+ACTCAC)GATCCTAGTACCCAAACAAAAGCTGGGCATT NGG 10 8 17 8 24 0 100 5 10
    240604231307_s522 GGGACAATGCCCAGCTTTTGTTTGGGTACTAG(+ACTCAC)GATCCAAATGGCAGTCTTCATGCTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240604231305_p20g GGACAGCTCACCATTATGATCTGTACCAGTTG(+ACTCAC)GTACCTGCCTTGGTCAGCAGGGAGCTGAGGA NGG 10 8 17 8 24 0 100 5 10
    240604231304_e9uv TATGATCCTCAGCTCCCTGCTGACCAAGGCAG(+ACTCAC)GTACCAACTGGTACAGATCATAATGGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240604231302_thuh GTTCACTAAGTAACTTGTGCTCATTTTCATTG(+ACTCAC)GATCCTGCAGTGACTCATTTCCATATGGTCG NGG 10 8 17 8 24 0 100 5 10
    240604231301_9bc7 CAATGCGACCATATGGAAATGAGTCACTGCAG(+ACTCAC)GATCCAATGAAAATGAGCACAAGTTACTTAG NGG 10 8 17 8 24 0 100 5 10
    240604231259_sjpy GCTACATCTCCCAGACTTTCAGTCTTATCTAG(+ACTCAC)GATCCTCTCCTCTTCCCAGCCTGAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240604231258_zyop TGGGGCCTGGGTTCAGGCTGGGAAGAGGAGAG(+ACTCAC)GATCCTAGATAAGACTGAAAGTCTGGGAGAT NGG 10 8 17 8 24 0 100 5 10