Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231226_ksy5 ATCAAATAGTTAAGAAGGAAATATTTCAAATG(+ACTCAC)GTACCAAATAAGACTCCTTTGTAAGCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240604231225_vqhe AAAAATCTAAGCTTACAAAGGAGTCTTATTTG(+ACTCAC)GTACCATTTGAAATATTTCCTTCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240604231224_4b5q ATCAAATAGTTAAGAAGGAAATATTTCAAATG(+ACTCAC)GTACCAAATAAGACTCCTTTGTAAGCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240604231222_kfft AAAAATCTAAGCTTACAAAGGAGTCTTATTTG(+ACTCAC)GTACCATTTGAAATATTTCCTTCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240604231221_1fr4 TTTAGTCACAATAAAGACCCCAAATGAATAAG(+ACTCAC)GTACCATGTGTGTATATATATACACATATAT NGG 10 8 17 8 24 0 100 5 10
    240604231220_mz0y TGTGTATATATGTGTATATATATACACACATG(+ACTCAC)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240604231218_g1qz TTTAGTCACAATAAAGACCCCAAATGAATAAG(+ACTCAC)GTACCATGTGTGTATATATATACACATATAT NGG 10 8 17 8 24 0 100 5 10
    240604231217_a0g0 TGTGTATATATGTGTATATATATACACACATG(+ACTCAC)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240604231215_r70d TTTAGTCACAATAAGGACCCCAAATGAATAAG(+ACTCAC)GTACCATGTGTGTGTGTGTGTGTGTGTATAC NGG 10 8 17 8 24 0 100 5 10
    240604231214_kdbf TATGTGTATACACACACACACACACACACATG(+ACTCAC)GTACCTTATTCATTTGGGGTCCTTATTGTGA NGG 10 8 17 8 24 0 100 5 10