Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231241_hme8 CTGTATCTACCCAGGCTATGAGCCAGGATCTG(+ACTCAC)GTACCACCCTCCTTCTGTACCAAGAACACTG NGG 10 8 17 8 24 0 100 5 10
    240604231239_f0nh TACCACAGTGTTCTTGGTACAGAAGGAGGGTG(+ACTCAC)GTACCAGATCCTGGCTCATAGCCTGGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240604231238_dth6 CCCAGCAGGGGCAGGAGAGGACTGGTCTCATG(+ACTCAC)GATCCCCTCTCCCCTGTTCTGCGGGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240604231236_yp9h CAGCGGAGGTGCCCGCAGAACAGGGGAGAGGG(+ACTCAC)GATCCATGAGACCAGTCCTCTCCTGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240604231234_18sq TTCTGTGTGTAGGGGTCTGTCCATGATGTTGG(+ACTCAC)GTACCACCTGAATTAATCCCGGATTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240604231233_ls2a ACACATAACAAAATCCGGGATTAATTCAGGTG(+ACTCAC)GTACCCAACATCATGGACAGACCCCTACACA NGG 10 8 17 8 24 0 100 5 10
    240604231231_hbcs ACACACAGCATGTAATTAACAACAGATCTATG(+ACTCAC)GATCCAGCTCTTAATATTGATCCTTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240604231230_czof AACACCACCAAAAGGATCAATATTAAGAGCTG(+ACTCAC)GATCCATAGATCTGTTGTTAATTACATGCTG NGG 10 8 17 8 24 0 100 5 10
    240604231229_ctx9 ATGTAGAGTCTCTTAGAGGACAGCCATACTAG(+ACTCAC)GATCCTATCTGCAAGCACAACATGGCATCAG NGG 10 8 17 8 24 0 100 5 10
    240604231228_6bwt TATTACTGATGCCATGTTGTGCTTGCAGATAG(+ACTCAC)GATCCTAGTATGGCTGTCCTCTAAGAGACTC NGG 10 8 17 8 24 0 100 5 10