Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231139_88cx CTCCTATGCAGACAGCTGCCATTCCTGCCAAG(+ACTCAC)GTACCTAGGCTGAGGTTCAGCCTCCCAGATC NGG 10 8 17 8 24 0 100 5 10
    240604231137_j8gu CCTTGGATCTGGGAGGCTGAACCTCAGCCTAG(+ACTCAC)GTACCTTGGCAGGAATGGCAGCTGTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240604231135_pmq5 AAGGCCACCAGCGAGGCCGAGCAAGGCCTCTG(+ACTCAC)GTACCCACTGACTACCCTAGAGCTCCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240604231133_hw6f CTCCCAAAGGGGAGCTCTAGGGTAGTCAGTGG(+ACTCAC)GTACCAGAGGCCTTGCTCGGCCTCGCTGGTG NGG 10 8 17 8 24 0 100 5 10
    240604231130_7ijr CCAATTAAATTAAAACCTTAGCTAGGACCCAG(+ACTCAC)GTACCAGTATGTTTATTGGGAGCCACTGATA NGG 10 8 17 8 24 0 100 5 10
    240604231129_h3dt CTATGTATCAGTGGCTCCCAATAAACATACTG(+ACTCAC)GTACCTGGGTCCTAGCTAAGGTTTTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240604231127_3zvh CTGGAGACCGTGGGGTCTAGGTAGCCTGCCAG(+ACTCAC)GATCCCAGCAGTGAGCTCACTTTGGGCCATT NGG 10 8 17 8 24 0 100 5 10
    240604231125_ez5i AATGAAATGGCCCAAAGTGAGCTCACTGCTGG(+ACTCAC)GATCCTGGCAGGCTACCTAGACCCCACGGTC NGG 10 8 17 8 24 0 100 5 10
    240604231124_8nl7 AAGGCTGAGATTAAAGAAAGGTTCCTGCTGTG(+ACTCAC)GTACCCAACTTTTTATCTGGGTGCTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240604231122_j9vb CACGGGGACACAGCACCCAGATAAAAAGTTGG(+ACTCAC)GTACCACAGCAGGAACCTTTCTTTAATCTCA NGG 10 8 17 8 24 0 100 5 10