Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231104_ea2f TGGCAAACCACTGTCTGCTATGGTTCTGAGAG(+ACTCAC)GATCCAGAGCCTTGGTATTCAAAAGCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240604231102_dg5x CCGACACTTGGCTTTTGAATACCAAGGCTCTG(+ACTCAC)GATCCTCTCAGAACCATAGCAGACAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240604231100_q9ep TGAAGTCCATTGCAGCTACAGTTGCTGATGGG(+ACTCAC)GATCCCATAGGAGAAAATGGACCAGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240604231059_9fq4 CGTTCTCTTCCCTGGTCCATTTTCTCCTATGG(+ACTCAC)GATCCCCATCAGCAACTGTAGCTGCAATGGA NGG 10 8 17 8 24 0 100 5 10
    240604231056_ldti AAGTCATACTAACACCACCTGGTGGAAACCGG(+ACTCAC)GTACCCATGACTCTGGGTACTAATAAAACCA NGG 10 8 17 8 24 0 100 5 10
    240604231055_l77c GGAGTTGGTTTTATTAGTACCCAGAGTCATGG(+ACTCAC)GTACCCGGTTTCCACCAGGTGGTGTTAGTAT NGG 10 8 17 8 24 0 100 5 10
    240604231053_xepf CTGGATGGATGGATGCCACCTTCTGGGAACGG(+ACTCAC)GATCCCTTAGCAGTGGAAATAATGGCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604231052_14eo GGTGCCTGAGGCCATTATTTCCACTGCTAAGG(+ACTCAC)GATCCCGTTCCCAGAAGGTGGCATCCATCCA NGG 10 8 17 8 24 0 100 5 10
    240604231050_kxg7 TTGAAATGTAAAGCCTGTCCATATGTTTTAAG(+ACTCAC)GATCCATGTATTTCTGTTTTGTTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604231049_mwpq GTTTTGAAAAACAAACAAAACAGAAATACATG(+ACTCAC)GATCCTTAAAACATATGGACAGGCTTTACAT NGG 10 8 17 8 24 0 100 5 10