Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604231015_m99s TGTCACCAGGGGGTGTGCAAGCCGGCATAAGG(+ACTCAC)GATCCCTTGGAGATACTCAAGAAGAGGGGCT NGG 10 8 17 8 24 0 100 5 10
    240604231013_gtd7 CAGGGAGCCCCTCTTCTTGAGTATCTCCAAGG(+ACTCAC)GATCCCTTATGCCGGCTTGCACACCCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240604231011_pbqr AGTCTTCAGCAACCACCATGGCCTGATATTGG(+ACTCAC)GTACCACCCTGCAGGTTATCAGCTACACTCC NGG 10 8 17 8 24 0 100 5 10
    240604231010_zkpf ATATGGGAGTGTAGCTGATAACCTGCAGGGTG(+ACTCAC)GTACCCAATATCAGGCCATGGTGGTTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240604231007_sdjd ACTGCTCTGAGCTTAGCACACTCCTGTCCCAG(+ACTCAC)GATCCTTCCTGCCTCCCATCCCCCATATCAC NGG 10 8 17 8 24 0 100 5 10
    240604231005_tstx AGCAGGTGATATGGGGGATGGGAGGCAGGAAG(+ACTCAC)GATCCTGGGACAGGAGTGTGCTAAGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240604231003_e746 AAGCAGTGGAAAGAGAACAGGTTGTTTCCCTG(+ACTCAC)GTACCTTAGTACTGTGGAACAAAAATAACCC NGG 10 8 17 8 24 0 100 5 10
    240604231001_ehxp TTTGGGGGTTATTTTTGTTCCACAGTACTAAG(+ACTCAC)GTACCAGGGAAACAACCTGTTCTCTTTCCAC NGG 10 8 17 8 24 0 100 5 10
    240604231000_sod8 AGTGTCACGCCTTGGGACCTCCCCTTGAGCTG(+ACTCAC)GATCCCATTTTGAGCCTGTCGCTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240604230959_j3q0 GAAAAGAAGGTCCAGCGACAGGCTCAAAATGG(+ACTCAC)GATCCAGCTCAAGGGGAGGTCCCAAGGCGTG NGG 10 8 17 8 24 0 100 5 10