Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230957_w1rm GAGGATTTTTGTTTTGGTTTGGTTTTAACTAG(+ACTCAC)GATCCTAACAAAAATGAGAGAACCAGCATTT NGG 10 8 17 8 24 0 100 5 10
    240604230956_lygm ACAAGAAATGCTGGTTCTCTCATTTTTGTTAG(+ACTCAC)GATCCTAGTTAAAACCAAACCAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240604230955_789l CGGACTGGCCAGCAGTAACGACGCTGCAACAG(+ACTCAC)GATCCTTCTGCACATGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240604230953_cp2o AATCAAGCTCTCCCAATAAACATGTGCAGAAG(+ACTCAC)GATCCTGTTGCAGCGTCGTTACTGCTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240604230951_7dr1 AACAATGCTAGCCTGGCCTTCTGGGTCCATGG(+ACTCAC)GTACCAGCTCTGGCACCGCCATCCCTGATCT NGG 10 8 17 8 24 0 100 5 10
    240604230950_0z0q CACTGAGATCAGGGATGGCGGTGCCAGAGCTG(+ACTCAC)GTACCCATGGACCCAGAAGGCCAGGCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240604230948_b0iz AGCACAGACACTCGTGTCTCACATCACACTGG(+ACTCAC)GTACCTAGGGGCGGATGTGGACACACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240604230946_fqg3 ATCTCCTTGGGTGTGTCCACATCCGCCCCTAG(+ACTCAC)GTACCCAGTGTGATGTGAGACACGAGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604230944_p8fz AATCGAGGGAAAAAATTACATATGACTGCTTG(+ACTCAC)GTACCTCTTCATTTGGCAAAGGCAGCCTCAA NGG 10 8 17 8 24 0 100 5 10
    240604230943_67os TAGTCTTGAGGCTGCCTTTGCCAAATGAAGAG(+ACTCAC)GTACCAAGCAGTCATATGTAATTTTTTCCCT NGG 10 8 17 8 24 0 100 5 10