Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230926_4vtm AGATAGAAAAATTGTCAGTACACTAGCTCTAG(+ACTCAC)GATCCAGAATGCAGGTGGCTGGTTTCACCCA NGG 10 8 17 8 24 0 100 5 10
    240604230924_u3y0 CCCTTTGGGTGAAACCAGCCACCTGCATTCTG(+ACTCAC)GATCCTAGAGCTAGTGTACTGACAATTTTTC NGG 10 8 17 8 24 0 100 5 10
    240604230923_55y9 GGTTTCATACCATTTGAGCCAGTGTCATGGTG(+ACTCAC)GTACCCATACTCTTTTGTTCAGCTCTCAGTC NGG 10 8 17 8 24 0 100 5 10
    240604230921_bo7r GACAGGACTGAGAGCTGAACAAAAGAGTATGG(+ACTCAC)GTACCACCATGACACTGGCTCAAATGGTATG NGG 10 8 17 8 24 0 100 5 10
    240604230920_myfl AACAAAAGAGCTTTGGGCCAGTTTGACAGTTG(+ACTCAC)GTACCCCCTCTAATGCCTCACCTAAATGGGG NGG 10 8 17 8 24 0 100 5 10
    240604230917_2ra0 AAACGCCCCATTTAGGTGAGGCATTAGAGGGG(+ACTCAC)GTACCAACTGTCAAACTGGCCCAAAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240604230916_snxw AGATTGTTATCAAGGAAACTCAGTAAGAATAG(+ACTCAC)GTACCCAAATAAATCGGGAAGTTTGTATCCT NGG 10 8 17 8 24 0 100 5 10
    240604230915_67iq GGGCTAGGATACAAACTTCCCGATTTATTTGG(+ACTCAC)GTACCTATTCTTACTGAGTTTCCTTGATAAC NGG 10 8 17 8 24 0 100 5 10
    240604230913_cuzt TACTATCTTTATTTAAAAATCAAAAACATTTG(+ACTCAC)GTACCTGAGGATAGCTGCAATGTGACTAATA NGG 10 8 17 8 24 0 100 5 10
    240604230911_7ftb CTTTTTATTAGTCACATTGCAGCTATCCTCAG(+ACTCAC)GTACCAAATGTTTTTGATTTTTAAATAAAGA NGG 10 8 17 8 24 0 100 5 10