Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230818_lc85 AGACCTGCCTGCCTCTGCCTCCCAAGTGCTGG(+ACTCAC)GATCCAAGGTGTGAGCCACAGCCTGGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240604230816_c23c AGGCCTGAAGCCAGGCTGTGGCTCACACCTTG(+ACTCAC)GATCCCAGCACTTGGGAGGCAGAGGCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604230814_3wb9 ACGAGAAATGTGGGTTCATTTAGTTTAATAGG(+ACTCAC)GATCCATGATTGAGGACTAGGGTGAGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240604230812_z7he CCTTTAACTACTCACCCTAGTCCTCAATCATG(+ACTCAC)GATCCCTATTAAACTAAATGAACCCACATTT NGG 10 8 17 8 24 0 100 5 10
    240604230811_smt1 CCCCATGTTAGTTGTAAAATATCAGCTGCTAG(+ACTCAC)GTACCCAGGTTTGCTTTGCAGGGCATATTTT NGG 10 8 17 8 24 0 100 5 10
    240604230808_h93n TCTATAAAATATGCCCTGCAAAGCAAACCTGG(+ACTCAC)GTACCTAGCAGCTGATATTTTACAACTAACA NGG 10 8 17 8 24 0 100 5 10
    240604230806_jf0e TTATTTCCCACGGTCTGCACAGCAGCTGGAGG(+ACTCAC)GTACCACAGCCCGACTGATGGCTACAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240604230805_0wca CCAGAGGCTTTGTAGCCATCAGTCGGGCTGTG(+ACTCAC)GTACCCTCCAGCTGCTGTGCAGACCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604230804_zadj TTTTTCTTACCTGTAAAATGAAGGTGTAGTAG(+ACTCAC)GTACCACCTAGTGCTGTGGGGAGCATCACAA NGG 10 8 17 8 24 0 100 5 10
    240604230802_j5eb TTTTCTTGTGATGCTCCCCACAGCACTAGGTG(+ACTCAC)GTACCTACTACACCTTCATTTTACAGGTAAG NGG 10 8 17 8 24 0 100 5 10