Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230800_lrz9 GACATTTTCTACCACTTAATGTAACATGCCTG(+ACTCAC)GTACCCGTCATGCAAAGCTGCAACACACAGC NGG 10 8 17 8 24 0 100 5 10
    240604230758_4836 AGTGGGCTGTGTGTTGCAGCTTTGCATGACGG(+ACTCAC)GTACCAGGCATGTTACATTAAGTGGTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240604230756_t9fk ACGCCCACCATTTATCTGAGTTTTTAAGGTAG(+ACTCAC)GTACCTTATTCCAGCACTCAGAAAGAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240604230755_v884 TTCTCCTGCCTCTTTCTGAGTGCTGGAATAAG(+ACTCAC)GTACCTACCTTAAAAACTCAGATAAATGGTG NGG 10 8 17 8 24 0 100 5 10
    240604230753_z6n8 CACCCACACCTGTCTGTACCTCACTCCAGAGG(+ACTCAC)GATCCACCGCCCTCTTCTGCCCTCTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240604230751_n8zo CAGGTGCCTGCAGAGGGCAGAAGAGGGCGGTG(+ACTCAC)GATCCCTCTGGAGTGAGGTACAGACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604230748_n2u3 CAGCTTTCCTCTCTTTACCTTTCACGCCTTGG(+ACTCAC)GATCCTAAGGATAAAGCCCTGGTTGGTAGTA NGG 10 8 17 8 24 0 100 5 10
    240604230746_6owy TTAGTTACTACCAACCAGGGCTTTATCCTTAG(+ACTCAC)GATCCCAAGGCGTGAAAGGTAAAGAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604230745_vzzw AAAACGGCAGCACCTCACGGCTGTCTTTCTAG(+ACTCAC)GATCCTCCATTATTCCTTAGAAGCAAGGACC NGG 10 8 17 8 24 0 100 5 10
    240604230743_sjee GACCAGGTCCTTGCTTCTAAGGAATAATGGAG(+ACTCAC)GATCCTAGAAAGACAGCCGTGAGGTGCTGCC NGG 10 8 17 8 24 0 100 5 10