Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230834_b7fh GGATGGACCATCTAGTGATTGCCATATCCAGG(+ACTCAC)GATCCATCCCATAATCAGCTTCCAAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240604230832_qem5 GTGTCAGCCTTTTGGAAGCTGATTATGGGATG(+ACTCAC)GATCCCTGGATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240604230831_hd0i AACTCCTGGCCCTTCCATGAACATTCAAAAAG(+ACTCAC)GATCCACTTGTGTTCTAGAACGAGCGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604230830_x9ph GTTCCTACAGCGCTCGTTCTAGAACACAAGTG(+ACTCAC)GATCCTTTTTGAATGTTCATGGAAGGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240604230828_atwq CTTATAGCTGTTGAGTATTTTCAATATCACTG(+ACTCAC)GATCCCTCAGTCTTCACCCATTGGTAAATAT NGG 10 8 17 8 24 0 100 5 10
    240604230826_hvle CTTATATATTTACCAATGGGTGAAGACTGAGG(+ACTCAC)GATCCAGTGATATTGAAAATACTCAACAGCT NGG 10 8 17 8 24 0 100 5 10
    240604230825_3ldh CAGTATACTATGATCAAAGTGAGACGATAAAG(+ACTCAC)GATCCTGCAATCTAAAGAGACAGAAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240604230823_ktg5 TTCACTGTTACTTCTGTCTCTTTAGATTGCAG(+ACTCAC)GATCCTTTATCGTCTCACTTTGATCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240604230821_3kem TGGGAGTGTTTCCTGCCATGCATGAAGCCTTG(+ACTCAC)GATCCACCCCAGCGTTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604230819_5hcv CTCCCACACCCAGTTCACACAACGCTGGGGTG(+ACTCAC)GATCCAAGGCTTCATGCATGGCAGGAAACAC NGG 10 8 17 8 24 0 100 5 10