Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230721_ir7z GAAAGAGACAAAAGCTGAACTATCCCTTAGTG(+ACTCAC)GATCCCCACTTATGTGCCTGGGTGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604230719_s4yc AGAGTTCTTTCTCACCCAGGCACATAAGTGGG(+ACTCAC)GATCCACTAAGGGATAGTTCAGCTTTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604230717_rhg6 AAAAAAAAAAGTCCTGGTCTAATGATTATCTG(+ACTCAC)GATCCAGCCTAATTCTCAGATTCTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240604230715_j2he TAGTGATTCTTGAGAATCTGAGAATTAGGCTG(+ACTCAC)GATCCAGATAATCATTAGACCAGGACTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604230713_n0b4 AAGGTAGATGCCAAACACCAGCATCTCACTGG(+ACTCAC)GATCCACAATCAGGAAATACTTCAGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240604230712_bze1 CCTGGCCAGTGCTGAAGTATTTCCTGATTGTG(+ACTCAC)GATCCCAGTGAGATGCTGGTGTTTGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240604230710_bkym TTTGCAGGAGTTGGTTCTCCTTGAACTGAGTG(+ACTCAC)GATCCTAGGGATTAAACGCCTGTCACTGTGT NGG 10 8 17 8 24 0 100 5 10
    240604230707_6rdh CCTCAACACAGTGACAGGCGTTTAATCCCTAG(+ACTCAC)GATCCACTCAGTTCAAGGAGAACCAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240604230705_hvx4 GACGGGCACGGTGAGCTAGGACAAGGAACGAG(+ACTCAC)GATCCAGGAGTCTGTGTAGGAATTAGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240604230703_8zbu CACCCATCTTCTAATTCCTACACAGACTCCTG(+ACTCAC)GATCCTCGTTCCTTGTCCTAGCTCACCGTGC NGG 10 8 17 8 24 0 100 5 10