Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230625_dzzv GTGAGTAGTAGGAACGAGATTCTTCATCCATG(+ACTCAC)GTACCCTTATCTAATTATGGGATTCGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240604230624_544t CCAGCCAGAGCGAATCCCATAATTAGATAAGG(+ACTCAC)GTACCATGGATGAAGAATCTCGTTCCTACTA NGG 10 8 17 8 24 0 100 5 10
    240604230623_l56o CTCCAGCGGCTTCCACAGTGGGCCTTCTGTAG(+ACTCAC)GATCCACAGGAGTCAAGACCTGAGGCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240604230620_qv8t CTTGGACCTGGCCTCAGGTCTTGACTCCTGTG(+ACTCAC)GATCCTACAGAAGGCCCACTGTGGAAGCCGC NGG 10 8 17 8 24 0 100 5 10
    240604230618_4iqv TGCTGGGGAAAGAGGGTTGTGCTCTCCCTAGG(+ACTCAC)GTACCTGTCTCTCCACCTCATCCCAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240604230617_ag01 AGCTCTGTGCTTGGGATGAGGTGGAGAGACAG(+ACTCAC)GTACCCTAGGGAGAGCACAACCCTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604230616_9xlp CAGCAAACTTTCAACATCTGGAAAATCAGCTG(+ACTCAC)GTACCTACTTCAGAGCACTAGCAGGACATTT NGG 10 8 17 8 24 0 100 5 10
    240604230614_xw2d GAATAAAATGTCCTGCTAGTGCTCTGAAGTAG(+ACTCAC)GTACCAGCTGATTTTCCAGATGTTGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240604230613_cn1e TGTCCACTATATAACAGGGTGTCATGGACAAG(+ACTCAC)GATCCTCTTAGTTATTTGCTTTGAATGTGAA NGG 10 8 17 8 24 0 100 5 10
    240604230612_bcjq TCAAGTTCACATTCAAAGCAAATAACTAAGAG(+ACTCAC)GATCCTTGTCCATGACACCCTGTTATATAGT NGG 10 8 17 8 24 0 100 5 10