Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230519_denq GTGTTATATGTTAAAACTTTCTCTTTAGCAAG(+ACTCAC)GTACCAAGAAATTCACCTGTTCCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240604230517_rxyl GCCCATATCTGAAGGAACAGGTGAATTTCTTG(+ACTCAC)GTACCTTGCTAAAGAGAAAGTTTTAACATAT NGG 10 8 17 8 24 0 100 5 10
    240604230515_v2v9 AGATTATATAGAAACAATCTGTACTGCTCTGG(+ACTCAC)GATCCTTTGGTCTTAGAAACCATTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604230513_rozi CACCCCCCCAAAAATGGTTTCTAAGACCAAAG(+ACTCAC)GATCCCAGAGCAGTACAGATTGTTTCTATAT NGG 10 8 17 8 24 0 100 5 10
    240604230512_7qhf TTCATGATTTCTTATCATGTTACCATAGCAAG(+ACTCAC)GATCCAGGTCCTTGCTGGAGTCAGTGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240604230510_7en1 GTCACATGACCACTGACTCCAGCAAGGACCTG(+ACTCAC)GATCCTTGCTATGGTAACATGATAAGAAATC NGG 10 8 17 8 24 0 100 5 10
    240604230509_41sk ATTAGTGTAAAGGTATCTCACCCTTAGATCTG(+ACTCAC)GATCCCTTGGAACTGGAGTTACAATTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240604230506_m0ch GGCAGCTTACAATTGTAACTCCAGTTCCAAGG(+ACTCAC)GATCCAGATCTAAGGGTGAGATACCTTTACA NGG 10 8 17 8 24 0 100 5 10
    240604230505_bino GTAACAGACCATGTGAAAGCTCACACTGCTTG(+ACTCAC)GATCCACTGCCTGGGCGGGCGGCTCCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240604230503_b5zi ACAGCAGCCTGGAGCCGCCCGCCCAGGCAGTG(+ACTCAC)GATCCAAGCAGTGTGAGCTTTCACATGGTCT NGG 10 8 17 8 24 0 100 5 10