Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230501_tb55 TGTAAGAATGTGGCCAAGGTGGTACAGCCTTG(+ACTCAC)GTACCATAATGCAAGACACAGTGACAATGTC NGG 10 8 17 8 24 0 100 5 10
    240604230500_qnbd AACTCGACATTGTCACTGTGTCTTGCATTATG(+ACTCAC)GTACCAAGGCTGTACCACCTTGGCCACATTC NGG 10 8 17 8 24 0 100 5 10
    240604230459_wnbc CCAGGCCACAAACCTGCCTGTGGGGCACTGAG(+ACTCAC)GATCCTAGAGGATGCCATCTTCAGGTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240604230457_gtnr CTCCTCCCCTACCTGAAGATGGCATCCTCTAG(+ACTCAC)GATCCTCAGTGCCCCACAGGCAGGTTTGTGG NGG 10 8 17 8 24 0 100 5 10
    240604230456_c9d2 GGATGGAACTTGAGAATATCATCCTGAGTGAG(+ACTCAC)GTACCCCGGAGCCAGCAAGACAGATGATATA NGG 10 8 17 8 24 0 100 5 10
    240604230453_jqar GAGTATATATCATCTGTCTTGCTGGCTCCGGG(+ACTCAC)GTACCTCACTCAGGATGATATTCTCAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604230451_dxs5 AACTAAGCCACTGAAATCGAATTGAATCAGGG(+ACTCAC)GATCCCAAATAAAAAGATCATATTAAGGCAA NGG 10 8 17 8 24 0 100 5 10
    240604230449_s06e AGAATTTGCCTTAATATGATCTTTTTATTTGG(+ACTCAC)GATCCCCTGATTCAATTCGATTTCAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604230447_pq2h TGGATCCCACAAGGGACCACCAAGTGACCTGG(+ACTCAC)GTACCTCAGTCATGTTAAAACATGTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240604230445_re3t AGGAAAGGAAAACATGTTTTAACATGACTGAG(+ACTCAC)GTACCCAGGTCACTTGGTGGTCCCTTGTGGG NGG 10 8 17 8 24 0 100 5 10