Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230352_p4gv TGGGTGCCCATAGAGGTTACAAGGGGCCATTG(+ACTCAC)GATCCTTTTGACCTGGAGTTGAGGAATGTTA NGG 10 8 17 8 24 0 100 5 10
    240604230351_xccq AAAACTAACATTCCTCAACTCCAGGTCAAAAG(+ACTCAC)GATCCAATGGCCCCTTGTAACCTCTATGGGC NGG 10 8 17 8 24 0 100 5 10
    240604230348_xn2k TCTAGAAACATGTCGATGGAAGAGAAACTTTG(+ACTCAC)GATCCCATGCCTCCTGTTTACAGTAGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240604230347_fsbv AGAAGAAAAGCTACTGTAAACAGGAGGCATGG(+ACTCAC)GATCCAAAGTTTCTCTTCCATCGACATGTTT NGG 10 8 17 8 24 0 100 5 10
    240604230346_ee9o AGTAACTTTGCAGTTTTTGTAGTTAAAATATG(+ACTCAC)GATCCAAAAGGTATATATGCATGGCGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240604230344_jrgb GACCAGGACACGCCATGCATATATACCTTTTG(+ACTCAC)GATCCATATTTTAACTACAAAAACTGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240604230343_dstp CATCCTAAACACAGCTTCTCTGTGTGTAAAAG(+ACTCAC)GTACCTGCTATTCCTAAGGGGCTGCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240604230341_e7pg ATGTTGCCTGTGCAGCCCCTTAGGAATAGCAG(+ACTCAC)GTACCTTTTACACACAGAGAAGCTGTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240604230340_nr7j TGAAGACTAACTGCTTCTTTGAAGGATTAAAG(+ACTCAC)GATCCCAGCCTAGAACTCATCTGGAGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604230338_02qr ATGCTCCTGACTCCAGATGAGTTCTAGGCTGG(+ACTCAC)GATCCTTTAATCCTTCAAAGAAGCAGTTAGT NGG 10 8 17 8 24 0 100 5 10