Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230443_pphu TTCATTTATGATTGGCTAACAAGTAACACCTG(+ACTCAC)GATCCCACAAGGGACCACCAAGTGACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604230441_bd2s AGGTACCCAGGTCACTTGGTGGTCCCTTGTGG(+ACTCAC)GATCCAGGTGTTACTTGTTAGCCAATCATAA NGG 10 8 17 8 24 0 100 5 10
    240604230439_rtf6 TGTCTTTTGGCTAATCCTAAGTAGCTCCTTTG(+ACTCAC)GATCCTCCTTCACAGGCTCTGTAGCTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240604230437_le2k AGCCATCTACAGCTACAGAGCCTGTGAAGGAG(+ACTCAC)GATCCAAAGGAGCTACTTAGGATTAGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240604230436_dwvq TCGCAAAACTTGGTCTCTGCTGGAGAACACTG(+ACTCAC)GATCCCCTGTGGGTGGGTGGGTGGGCTGTTG NGG 10 8 17 8 24 0 100 5 10
    240604230433_52gm ATTTTCAACAGCCCACCCACCCACCCACAGGG(+ACTCAC)GATCCAGTGTTCTCCAGCAGAGACCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240604230432_g9fs AGAGAGCCCTTTACATCCACTCTGTTGGGCAG(+ACTCAC)GATCCAGTCAACCTGCTCAGCCTGCTTAGGC NGG 10 8 17 8 24 0 100 5 10
    240604230431_93jt GCTGAGCCTAAGCAGGCTGAGCAGGTTGACTG(+ACTCAC)GATCCTGCCCAACAGAGTGGATGTAAAGGGC NGG 10 8 17 8 24 0 100 5 10
    240604230428_bfl5 TGTTCCCCCATTGCACACTGGAGAAGCCCTGG(+ACTCAC)GTACCGAGTGTGGGGACCCCATGGACAGTAC NGG 10 8 17 8 24 0 100 5 10
    240604230426_w01s TTATGGTACTGTCCATGGGGTCCCCACACTCG(+ACTCAC)GTACCCAGGGCTTCTCCAGTGTGCAATGGGG NGG 10 8 17 8 24 0 100 5 10