Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230408_1b2e GGTACTGTTTTGTACTATATAATGACTTAGAG(+ACTCAC)GTACCAGGATGATGTCCTGGAAACACGGGTC NGG 10 8 17 8 24 0 100 5 10
    240604230406_s4a6 TATCAGACCCGTGTTTCCAGGACATCATCCTG(+ACTCAC)GTACCTCTAAGTCATTATATAGTACAAAACA NGG 10 8 17 8 24 0 100 5 10
    240604230405_crxv ACTAGATATCCACATATTAGAGAATGAAACTG(+ACTCAC)GATCCCTAAAATGTACCACATGCAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240604230403_f4zs TTGATTCATTTTTGCATGTGGTACATTTTAGG(+ACTCAC)GATCCAGTTTCATTCTCTAATATGTGGATAT NGG 10 8 17 8 24 0 100 5 10
    240604230402_89nq TTAGAACTTTTAAAAAATAAACTTTCTTTTTG(+ACTCAC)GTACCTCTGGCTTCCTATATATGTCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240604230400_jhlt AATGTTTCCAAGACATATATAGGAAGCCAGAG(+ACTCAC)GTACCAAAAAGAAAGTTTATTTTTTAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240604230359_qhb7 GGTAACTTCATTACAGTGTCTATAAAGTTGTG(+ACTCAC)GATCCCAGTCTTGGTGCCAAATGCATATAAA NGG 10 8 17 8 24 0 100 5 10
    240604230357_t0pn AGCTTTTTATATGCATTTGGCACCAAGACTGG(+ACTCAC)GATCCACAACTTTATAGACACTGTAATGAAG NGG 10 8 17 8 24 0 100 5 10
    240604230356_fo3l AGGATTCATAGTTGATCATCTTCCAGTTGATG(+ACTCAC)GTACCAACTAGATCTTATAAGTACTAGATAT NGG 10 8 17 8 24 0 100 5 10
    240604230354_jad2 TGTGGATATCTAGTACTTATAAGATCTAGTTG(+ACTCAC)GTACCATCAACTGGAAGATGATCAACTATGA NGG 10 8 17 8 24 0 100 5 10