Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604230533_y1jq CTTGGATGTTGGGAGTGTAGCAGGCACAGGAG(+ACTCAC)GTACCTCTGCAGAAGGAAGGCGGAGGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604230532_bf9v AGGCCTCCACCCTCCGCCTTCCTTCTGCAGAG(+ACTCAC)GTACCTCCTGTGCCTGCTACACTCCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240604230531_ymb3 TGTCCGTTTCCCCTCACTGTCAGGGAAAGCTG(+ACTCAC)GATCCCTCTGGCTGACTTAGAGTACTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240604230529_7lub CCCAAGTCACAGTACTCTAAGTCAGCCAGAGG(+ACTCAC)GATCCAGCTTTCCCTGACAGTGAGGGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604230528_j6st GCCGCTGGCTGGGTGGCCCAGGGGGGCTGGCG(+ACTCAC)GTACCGCCATGCTGGTTCCTTGGGGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240604230526_idiu TTCCAGAGGCCCCCCAAGGAACCAGCATGGCG(+ACTCAC)GTACCGCCAGCCCCCCTGGGCCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240604230525_bbet GAGCCAGAGTTGGGGATCTGAGCCCATGAATG(+ACTCAC)GTACCGCTTAGACCTCAGGCCAAAACACACC NGG 10 8 17 8 24 0 100 5 10
    240604230523_w6ig GGGAGGGTGTGTTTTGGCCTGAGGTCTAAGCG(+ACTCAC)GTACCATTCATGGGCTCAGATCCCCAACTCT NGG 10 8 17 8 24 0 100 5 10
    240604230522_ljnh CCAATCAGACCTAGATCCTGGCTGCTCTAGTG(+ACTCAC)GTACCAAGGTGTCCTGAGATATGGATGTTGT NGG 10 8 17 8 24 0 100 5 10
    240604230520_ns9u TGCACACAACATCCATATCTCAGGACACCTTG(+ACTCAC)GTACCACTAGAGCAGCCAGGATCTAGGTCTG NGG 10 8 17 8 24 0 100 5 10