Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160204_x6rt GATGTGTGTAGCCTGTGGTTTGTCTAGATTGG(+AGGACA)GTACCACTTATGTGGAAAGGGCACACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613160202_ijrn TTTAGACAAAAGTTGGTGCTGCTGTGGCGCAG(+AGGACA)GATCCTGGACTGTGGGGAGGGGAGGAGCCCT NGG 10 8 17 8 24 0 100 5 10
    240613160201_sp67 ACCCCAGGGCTCCTCCCCTCCCCACAGTCCAG(+AGGACA)GATCCTGCGCCACAGCAGCACCAACTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613160159_327k ATAAAAATTATACACGAGACCTGCTGTTTTGG(+AGGACA)GTACCTGGCTACTGCCCAGAGTGCAATGAGA NGG 10 8 17 8 24 0 100 5 10
    240613160157_khap ATGTTTCTCATTGCACTCTGGGCAGTAGCCAG(+AGGACA)GTACCCAAAACAGCAGGTCTCGTGTATAATT NGG 10 8 17 8 24 0 100 5 10
    240613160156_o75r CTCAGCAAGCTGGGATGTAGTGAAGCCTCGAG(+AGGACA)GATCCCCACTGTCAGAGGCTCTCTTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240613160154_o7ng TCCTGAACCAAAAGAGAGCCTCTGACAGTGGG(+AGGACA)GATCCTCGAGGCTTCACTACATCCCAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613160153_0kwb CTGCCCCAGATTCCTCCCCATCACCTCTCTGG(+AGGACA)GTACCTGATTTCCTGGTCTGTGCACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613160151_rw2c CCAAGGCTGCAGTGCACAGACCAGGAAATCAG(+AGGACA)GTACCCAGAGAGGTGATGGGGAGGAATCTGG NGG 10 8 17 8 24 0 100 5 10
    240613160150_bbym TGACTCCCCAGTGGAGGCAAGCCAGAGGTCTG(+AGGACA)GATCCTCCACCCTCTCCTGGAGTGCCCCAGA NGG 10 8 17 8 24 0 100 5 10