Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160217_cee5 CCAGTTCAGGCTGGGATGCACATTGCCCCCAG(+AGGACA)GTACCCCTGCTCAGTTCTAAAACAGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613160216_s8q0 TCCCCTGTGAGTAGTTAGAAAATACAAAAGCG(+AGGACA)GATCCAGTGCGGGACGTTGTGTGCAAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613160214_7vul CCTTCTCTCTTTGCACACAACGTCCCGCACTG(+AGGACA)GATCCGCTTTTGTATTTTCTAACTACTCACA NGG 10 8 17 8 24 0 100 5 10
    240613160213_70d4 AGCTGGGATGTTGTGGCCCTAATCCTGGCTTG(+AGGACA)GTACCCAGCACCCCCAGATCCTGCCAGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613160212_kmyd GCTTCAGCACTGGCAGGATCTGGGGGTGCTGG(+AGGACA)GTACCAAGCCAGGATTAGGGCCACAACATCC NGG 10 8 17 8 24 0 100 5 10
    240613160210_ol10 AGGACGGGTTAGGACAGACAGTATCTGTCCTG(+AGGACA)GATCCCAGTGGAAGTCAGGGGGCTGTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613160209_11i8 CATGCCAAGAACAGCCCCCTGACTTCCACTGG(+AGGACA)GATCCAGGACAGATACTGTCTGTCCTAACCC NGG 10 8 17 8 24 0 100 5 10
    240613160207_tetc CCTCAGTAACCCTGCATGTGTAATGGGCTGTG(+AGGACA)GTACCATCACACTGCCCATGTGCCGTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240613160206_0iq9 AGTCAGAGCCACGGCACATGGGCAGTGTGATG(+AGGACA)GTACCACAGCCCATTACACATGCAGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240613160205_8l42 CAATTATTTGGTGTGCCCTTTCCACATAAGTG(+AGGACA)GTACCCAATCTAGACAAACCACAGGCTACAC NGG 10 8 17 8 24 0 100 5 10