Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160324_9qhx AGATGTCACTCAAGCTGAAATCCACTGTCGAG(+AGGACA)GTACCCACCCCCTAAGAGCTACTGCCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240613160323_666w TGCTGAGATGGTGATGTTACTCTCCATTTTTG(+AGGACA)GTACCAGTTCTGAGACTGTAAGACTTTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613160322_ts6v CCCAGATGAAAAGTCTTACAGTCTCAGAACTG(+AGGACA)GTACCAAAAATGGAGAGTAACATCACCATCT NGG 10 8 17 8 24 0 100 5 10
    240613160320_k5h7 TTTTTGTGAGGGAGTTGATCCTGGGCAGTTAG(+AGGACA)GTACCCTGGAAGGAGGGGAGGGAACCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240613160318_fz1w GCTTCATCGAGGTTCCCTCCCCTCCTTCCAGG(+AGGACA)GTACCTAACTGCCCAGGATCAACTCCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240613160317_b3na ACCGGACCTTGTGGCCAAGTAGGATTCTGAGG(+AGGACA)GATCCAGACGAGAAGCCCAAAGAGTTCAGAT NGG 10 8 17 8 24 0 100 5 10
    240613160316_5juj CCGGCATCTGAACTCTTTGGGCTTCTCGTCTG(+AGGACA)GATCCCTCAGAATCCTACTTGGCCACAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613160314_2p8o AGTCCTTAGCAGCCCCCACCACCCACGCATCG(+AGGACA)GATCCCATTCTCAACCAGGACCCCCGCACCT NGG 10 8 17 8 24 0 100 5 10
    240613160313_du8x TGGACAGGTGCGGGGGTCCTGGTTGAGAATGG(+AGGACA)GATCCGATGCGTGGGTGGTGGGGGCTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240613160310_i72m GTCATCTTGTCAGTTCCTAACTGGAACCTTGG(+AGGACA)GATCCTTCTTCTTCCTCTCTTTCTTTCTTTC NGG 10 8 17 8 24 0 100 5 10