Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160339_ckji CTCAGTGAACTCATTCATAGGAAACCAGCCCG(+AGGACA)GTACCGGATGAGCTCAACTTTCGAATGAACT NGG 10 8 17 8 24 0 100 5 10
    240613160338_xj1c GGGGCCCCCTGCCCGCGCCCAGACACTTGATG(+AGGACA)GATCCCCACCAACGCCCCCCCTACCCCCACC NGG 10 8 17 8 24 0 100 5 10
    240613160336_r0eq GAGGTGGTGGGGGTAGGGGGGGCGTTGGTGGG(+AGGACA)GATCCATCAAGTGTCTGGGCGCGGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613160335_8kqa CTCTGGACCACTGAGCCATCTCCAACTTCTTG(+AGGACA)GATCCTTGGGACAACAAAAGGAGAGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613160333_y3lm AGAGTGGCAGCCTCTCCTTTTGTTGTCCCAAG(+AGGACA)GATCCAAGAAGTTGGAGATGGCTCAGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240613160331_ytw6 AGCGAACGCGTGTAAGACACTACTACACTCAG(+AGGACA)GATCCACAAGCTGTTGATTCCAAATTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613160330_vhtk CTCAGTCTGGAATTTGGAATCAACAGCTTGTG(+AGGACA)GATCCTGAGTGTAGTAGTGTCTTACACGCGT NGG 10 8 17 8 24 0 100 5 10
    240613160329_wsbk TATGGACACCTGTAGGACAGAAGAAGGTATTG(+AGGACA)GATCCCATTACAGATGGTTGTAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613160327_lzz5 ACCCCATGGTGGCTTACAACCATCTGTAATGG(+AGGACA)GATCCAATACCTTCTTCTGTCCTACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240613160325_142i AGTGGCCCTGGGCAGTAGCTCTTAGGGGGTGG(+AGGACA)GTACCTCGACAGTGGATTTCAGCTTGAGTGA NGG 10 8 17 8 24 0 100 5 10