Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160356_wyus TCCTAAGTCTTCTTTCTTTTTATTTAGCCCAG(+AGGACA)GATCCTAGCCCAGGAGATGGTGCTAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613160354_4wp9 TCCTCAGAAAATTGGACATAGTACTACCAGAG(+AGGACA)GATCCTGCAATACCTCTCCTGGGCATAGATC NGG 10 8 17 8 24 0 100 5 10
    240613160353_kpka TTCTGGATCTATGCCCAGGAGAGGTATTGCAG(+AGGACA)GATCCTCTGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613160351_w7kl GGATGGACCATCTAGAGACTGCCATATCCACG(+AGGACA)GATCCATCCCATAATTAGCCTCCAAACGATG NGG 10 8 17 8 24 0 100 5 10
    240613160350_wu7h GGTGTCATCGTTTGGAGGCTAATTATGGGATG(+AGGACA)GATCCGTGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613160348_1jfm CATCTTCATGAGTTGCTCTGTTATTTTGCAGG(+AGGACA)GATCCTGGGCAGCTTTGGCATTATCATGCAC NGG 10 8 17 8 24 0 100 5 10
    240613160346_35c7 GGAGTGTGCATGATAATGCCAAAGCTGCCCAG(+AGGACA)GATCCCTGCAAAATAACAGAGCAACTCATGA NGG 10 8 17 8 24 0 100 5 10
    240613160345_e1tb GCAACCCAATAATACTATCATAATCCGGACAG(+AGGACA)GATCCTCCCCGACTGTGGAGAGAGACCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613160343_lpg5 TTTCTCCAGGGTCTCTCTCCACAGTCGGGGAG(+AGGACA)GATCCTGTCCGGATTATGATAGTATTATTGG NGG 10 8 17 8 24 0 100 5 10
    240613160341_t3oo TTGGTAGTTCATTCGAAAGTTGAGCTCATCCG(+AGGACA)GTACCGGGCTGGTTTCCTATGAATGAGTTCA NGG 10 8 17 8 24 0 100 5 10