Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160456_3rqw AGCAGCATTCCTTTGCTTACCAAAATCTGCCG(+AGGACA)GTACCATGCCTTCCTCTAAGAGGGGAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613160455_x37y GGAAGCCAGGGCAGGGCAGAGCTCAGGTGATG(+AGGACA)GTACCTTGTGACAAGCACAGGTTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613160453_kq7r GATGATCTGAAGAAACCTGTGCTTGTCACAAG(+AGGACA)GTACCATCACCTGAGCTCTGCCCTGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613160452_tng5 CAACCATTTCCCCTTCCTGGATCCTCTGTCTG(+AGGACA)GTACCAAGCAATATATTTTTTTATTTTACCC NGG 10 8 17 8 24 0 100 5 10
    240613160451_24o9 TGCAAGGGTAAAATAAAAAAATATATTGCTTG(+AGGACA)GTACCAGACAGAGGATCCAGGAAGGGGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613160450_gqhx TACCAACCTTGCTAGCCTGTGGGGACTTTAAG(+AGGACA)GTACCGTGATGCATTTCATAAGGCTAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613160449_mppy CATATTCCCCTAGCCTTATGAAATGCATCACG(+AGGACA)GTACCTTAAAGTCCCCACAGGCTAGCAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613160447_qohd ATTACCAACTGCTGTCTCTCTGCTGTGAAAAG(+AGGACA)GTACCGTAATTCACGTGATCCATCTATACGT NGG 10 8 17 8 24 0 100 5 10
    240613160446_9vtv GTCACACGTATAGATGGATCACGTGAATTACG(+AGGACA)GTACCTTTTCACAGCAGAGAGACAGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613160445_k52v GAGAACCTAACCTTAGTTTCAGGTCAAGGTTG(+AGGACA)GTACCTCCTGGTGGAAATTCTCATTTGGATC NGG 10 8 17 8 24 0 100 5 10