Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160543_s3jc AACATCTTGTTCCTATATTTCTTCTGTGTAAG(+AGGACA)GTACCTGGAGGGGATTTAGTTCTACTGAACC NGG 10 8 17 8 24 0 100 5 10
    240613160541_qmke GCTCACAACCATCAGTAACTCTCATTCCAGGG(+AGGACA)GATCCGGCACCTTCTTTTCACCTCTTCGTGT NGG 10 8 17 8 24 0 100 5 10
    240613160539_34t9 CTGGTACACGAAGAGGTGAAAAGAAGGTGCCG(+AGGACA)GATCCCCTGGAATGAGAGTTACTGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613160538_woq7 TTTGAATGTGCACACCATGCGTGTGCCTGTTG(+AGGACA)GATCCCCTGGAACTGGGCTTGCGGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613160535_synj GCTCACAACCATCCGCAAGCCCAGTTCCAGGG(+AGGACA)GATCCAACAGGCACACGCATGGTGTGCACAT NGG 10 8 17 8 24 0 100 5 10
    240613160534_lgat AAGGGGCCCCCCACTCTACCCTGGGGAACTTG(+AGGACA)GATCCTCTTCAGAACCCTACCATGTTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613160533_03td ACACACAGACAACATGGTAGGGTTCTGAAGAG(+AGGACA)GATCCAAGTTCCCCAGGGTAGAGTGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613160531_sqh8 AATCCCAGCACTTAAGGGGGTGGAGGTAAGAG(+AGGACA)GATCCTGAGTTCAAGGCCAGCCTGGGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613160530_ugtw TAATGTTAAGCCCAGGCTGGCCTTGAACTCAG(+AGGACA)GATCCTCTTACCTCCACCCCCTTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613160528_m2pu GAGGCTAATACCAAACCTATAGCTACTCCTGG(+AGGACA)GTACCCTGCCTCCAGGTGATCACAGCCATCC NGG 10 8 17 8 24 0 100 5 10