Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160732_0zfl TACATCCCCAGCCAGCCTTTGTCTTTCTGTGG(+AGGACA)GTACCTGTGGTGCTGGGACCCGAACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613160731_kbzb TATTCCTGAGGGTTCGGGTCCCAGCACCACAG(+AGGACA)GTACCCACAGAAAGACAAAGGCTGGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613160729_3skq ACCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCTCTTCTGGTGTGTCTGAAGACAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613160728_es8s CACCGTCACTGTCTTCAGACACACCAGAAGAG(+AGGACA)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613160727_sc3a TTGACCTCTTCGTCCTTTTGTTTTTAGATCTG(+AGGACA)GTACCACTGGGATGATGACCTGAGACCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613160725_bfr8 TCCAGTGGGGGTCTCAGGTCATCATCCCAGTG(+AGGACA)GTACCAGATCTAAAAACAAAAGGACGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613160724_blab CAGTCTGAGAATGAGTGTGTGTGAGTGAGGCG(+AGGACA)GTACCCACATTCTCAACTTCAAGTCATTGCA NGG 10 8 17 8 24 0 100 5 10
    240613160722_t74t GAAACTGCAATGACTTGAAGTTGAGAATGTGG(+AGGACA)GTACCGCCTCACTCACACACACTCATTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613160721_mgzg CTCTTCCACTTTGTAAGAGGATTTTTCTGCTG(+AGGACA)GATCCCTCCTCTATGCCATCCAGCTCTGACC NGG 10 8 17 8 24 0 100 5 10
    240613160719_cno2 CTGGAGGTCAGAGCTGGATGGCATAGAGGAGG(+AGGACA)GATCCAGCAGAAAAATCCTCTTACAAAGTGG NGG 10 8 17 8 24 0 100 5 10