Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160648_v9qc TGGTCCACAGGAAGCCCTGCTCTTTTTGTTAG(+AGGACA)GATCCAGGAGCCTGCAATGACTCGTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613160646_05pb CCATGAGTACTGGAGATACAGGCATAAGACAG(+AGGACA)GATCCATGCCTTCTTTCTACCATGGGAAACC NGG 10 8 17 8 24 0 100 5 10
    240613160645_227j CCCTGGGTTTCCCATGGTAGAAAGAAGGCATG(+AGGACA)GATCCTGTCTTATGCCTGTATCTCCAGTACT NGG 10 8 17 8 24 0 100 5 10
    240613160643_oc47 GGGTGCTCCTTATGGGATCTTCTACCCAGTGG(+AGGACA)GATCCTCCAGGTTCCTCTGTCCTGTCATCCC NGG 10 8 17 8 24 0 100 5 10
    240613160642_2ygc GGGGTGGGATGACAGGACAGAGGAACCTGGAG(+AGGACA)GATCCCACTGGGTAGAAGATCCCATAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613160640_mt8y TAATGTCCTGACCAGTAACTTACCTCTTCCTG(+AGGACA)GATCCATTCACACTGTTTCAGCCTTGTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613160639_wg6x CCCAGCACAACAAGGCTGAAACAGTGTGAATG(+AGGACA)GATCCAGGAAGAGGTAAGTTACTGGTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613160636_kogk CCCTGTGGGGGAAAGGTGGGGGTTGGGGCCTG(+AGGACA)GATCCAGGGGAGGAGGCCATGGTGAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613160633_zg3q AGTTTTCCACTTCACCATGGCCTCCTCCCCTG(+AGGACA)GATCCAGGCCCCAACCCCCACCTTTCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613160631_on6s GCTTTGATACCTGGTTCAAGTGACCATGGGGG(+AGGACA)GATCCCATAGTACCTGGAATGAGATTGCTGG NGG 10 8 17 8 24 0 100 5 10