Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160614_klb8 CCTCCTCTGCCCAGTCTTTCAAACGGACTCAG(+AGGACA)GATCCCCCAATCCGCATGCCAGTACCAGATT NGG 10 8 17 8 24 0 100 5 10
    240613160612_1amk TCAAATGACTGCAGCCCCAAGCACAGCCCTAG(+AGGACA)GATCCAAACCAGGAAGAAAACAAATATTATT NGG 10 8 17 8 24 0 100 5 10
    240613160611_nmlp CCAGGAATAATATTTGTTTTCTTCCTGGTTTG(+AGGACA)GATCCTAGGGCTGTGCTTGGGGCTGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613160609_qlnp TTGGGGGATGCCTGGGCCAGGGAGAGGCACTG(+AGGACA)GTACCAGCCGTCCAAGCTGACAGCCTTACCC NGG 10 8 17 8 24 0 100 5 10
    240613160608_a12t CTTAGGGGTAAGGCTGTCAGCTTGGACGGCTG(+AGGACA)GTACCAGTGCCTCTCCCTGGCCCAGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240613160606_w2kv GCACTGAGTTCTAGGCCTCCTCTTGTCTCCTG(+AGGACA)GATCCTCACTTGAATCCTGCAGCAGAACGGA NGG 10 8 17 8 24 0 100 5 10
    240613160605_7pcj GGTTGTCCGTTCTGCTGCAGGATTCAAGTGAG(+AGGACA)GATCCAGGAGACAAGAGGAGGCCTAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240613160603_f0lo AAGTGGCAAGAGATGGATTCTGAAAGGGGAGG(+AGGACA)GTACCTGAGGGGGTCTCACCTCCCTTGGTCC NGG 10 8 17 8 24 0 100 5 10
    240613160601_l36i GGCTTGGACCAAGGGAGGTGAGACCCCCTCAG(+AGGACA)GTACCCTCCCCTTTCAGAATCCATCTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240613160600_u3o9 CCTCCTCCCCTTTTGTCATGAGAGCTAAGCTG(+AGGACA)GATCCATAATCTCTGTCCACACTAAAGGCTG NGG 10 8 17 8 24 0 100 5 10