Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160412_8djk CTTCTGTATGTAATCTCCAGCAAAGATAAATG(+AGGACA)GATCCTGGGAGCTGGCATTGTAGGAAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613160411_9pi9 CTTCCCGGGAGCCGGTCCTCGTCAGGGCTCGG(+AGGACA)GATCCCCCGGGACGGAGCACGTTCCCCGCAG NGG 10 8 17 8 24 0 100 5 10
    240613160408_vnjk GAGTTCTGCGGGGAACGTGCTCCGTCCCGGGG(+AGGACA)GATCCCGAGCCCTGACGAGGACCGGCTCCCG NGG 10 8 17 8 24 0 100 5 10
    240613160407_rgiq CATGATGCAAGCTAGAAACCTTTGTACAGGAG(+AGGACA)GATCCCAGATGGTAGAGATGTTGGGCATATA NGG 10 8 17 8 24 0 100 5 10
    240613160405_sdxs GCACCTATATGCCCAACATCTCTACCATCTGG(+AGGACA)GATCCTCCTGTACAAAGGTTTCTAGCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240613160403_blxl CATAACTGTGTATTGGGAAGATGGGATGAAGG(+AGGACA)GATCCAGTCAGAAAAGAAGAGATATCGATAA NGG 10 8 17 8 24 0 100 5 10
    240613160402_uq6u TTTATTTATCGATATCTCTTCTTTTCTGACTG(+AGGACA)GATCCCTTCATCCCATCTTCCCAATACACAG NGG 10 8 17 8 24 0 100 5 10
    240613160400_skkn CCATTTTTGTTCACAACCATTTCCCCTTCCTG(+AGGACA)GATCCTCTGTCTGGTACCAAGCAATATATTT NGG 10 8 17 8 24 0 100 5 10
    240613160359_zimd TAAAAAAATATATTGCTTGGTACCAGACAGAG(+AGGACA)GATCCAGGAAGGGGAAATGGTTGTGAACAAA NGG 10 8 17 8 24 0 100 5 10
    240613160358_0909 CACCCTGGCTCTAGCACCATCTCCTGGGCTAG(+AGGACA)GATCCTGGGCTAAATAAAAAGAAAGAAGACT NGG 10 8 17 8 24 0 100 5 10