Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221817_64r0 CTGGGGGACCATAGATATGATGAAAATAAAAG(+ACTCAC)GTACCTGGAATTTTTGACACATGTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240604221815_c7vf TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+ACTCAC)GTACCTTTTATTTTCATCATATCTATGGTCC NGG 10 8 17 8 24 0 100 5 10
    240604221813_onpb ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+ACTCAC)GATCCTTCCAGGTCTCTTCTTATACATACAC NGG 10 8 17 8 24 0 100 5 10
    240604221811_4ty7 CTTGTGTGTATGTATAAGAAGAGACCTGGAAG(+ACTCAC)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240604221810_dea9 CTGGGGGCCCATAGGTATGATGAAAATAAAAG(+ACTCAC)GTACCTGGAATTTTTGACACATGTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240604221807_dq6y TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+ACTCAC)GTACCTTTTATTTTCATCATACCTATGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604221805_7xa2 ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+ACTCAC)GATCCTTCCAGGTCTCTTCTTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240604221804_z4k2 CTTGTGTGTATGTGTAAGAAGAGACCTGGAAG(+ACTCAC)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240604221801_vh63 CTGTGGAAGAGTTTCTATGTGAGATGAGGATG(+ACTCAC)GATCCCTCAGAGTACACACCCATCTCATGGC NGG 10 8 17 8 24 0 100 5 10
    240604221759_c0t1 AGCAAGCCATGAGATGGGTGTGTACTCTGAGG(+ACTCAC)GATCCATCCTCATCTCACATAGAAACTCTTC NGG 10 8 17 8 24 0 100 5 10