Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221907_j1f6 TTACACTGCTTCACAAAAGGACGGGGCATGAG(+ACTCAC)GATCCCCTTGTCTATTTATAAAAGCAATAAA NGG 10 8 17 8 24 0 100 5 10
    240604221905_3pdy GTCATTTTATTGCTTTTATAAATAGACAAGGG(+ACTCAC)GATCCTCATGCCCCGTCCTTTTGTGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240604221904_e4jh TGGGAATCATTGTCCAGGAGTAACTCCAGCAG(+ACTCAC)GATCCCAGGTCTCTGCAGAAGCAGGAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240604221901_vw89 CAAGCCACCTTCCTGCTTCTGCAGAGACCTGG(+ACTCAC)GATCCTGCTGGAGTTACTCCTGGACAATGAT NGG 10 8 17 8 24 0 100 5 10
    240604221900_16dm CTCTTCCCAAAGAGCCACTTACCTCTGGACAG(+ACTCAC)GTACCCTGCGTCTGGTTCACGGTGACGATCA NGG 10 8 17 8 24 0 100 5 10
    240604221858_pdds CAACATGATCGTCACCGTGAACCAGACGCAGG(+ACTCAC)GTACCTGTCCAGAGGTAAGTGGCTCTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240604221857_3h75 TTGTATGCTGACAACTTTAATAATGTAAGGCG(+ACTCAC)GATCCGGCTCACAAGCCGGGAGGCAGCACAG NGG 10 8 17 8 24 0 100 5 10
    240604221855_x7bp CTTGTCTGTGCTGCCTCCCGGCTTGTGAGCCG(+ACTCAC)GATCCGCCTTACATTATTAAAGTTGTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604221853_ptwy GCCAAGTGCTGCTCTGCAGTCCGTGAAGCACG(+ACTCAC)GTACCCGCATCCGGACCATCATGACTATAGG NGG 10 8 17 8 24 0 100 5 10
    240604221852_2hk4 CACTCCCTATAGTCATGATGGTCCGGATGCGG(+ACTCAC)GTACCGTGCTTCACGGACTGCAGAGCAGCAC NGG 10 8 17 8 24 0 100 5 10