Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221952_wycg AAGCATCATCCTGGTTCTCTGCCGGCTGCCAG(+ACTCAC)GTACCCTAGTGCCAAGCACGTCTGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604221950_uda0 AGCATCAGGCACCAGACGTGCTTGGCACTAGG(+ACTCAC)GTACCTGGCAGCCGGCAGAGAACCAGGATGA NGG 10 8 17 8 24 0 100 5 10
    240604221949_d0r1 CATTTTTATCTCACAGTTACACCCTCCTGTCG(+ACTCAC)GATCCTCCTCCAAGTCTGACCACTGACTGGG NGG 10 8 17 8 24 0 100 5 10
    240604221947_llci GCTAGCCCAGTCAGTGGTCAGACTTGGAGGAG(+ACTCAC)GATCCGACAGGAGGGTGTAACTGTGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240604221946_dzs1 TAGCTGTATCTTTCTCACTCAGGTAGAAATGG(+ACTCAC)GATCCACCCTGCTTGACCTTTTCCAGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240604221944_8f68 TTGGAACCTTCTGGAAAAGGTCAAGCAGGGTG(+ACTCAC)GATCCCATTTCTACCTGAGTGAGAAAGATAC NGG 10 8 17 8 24 0 100 5 10
    240604221942_ygnz ACAGGTGTGCACTGCCAGTCCTGGGGGCTAAG(+ACTCAC)GATCCAACCCAGAGCACTGTCTGTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240604221941_pnuk TGGCACAGACAGACAGACAGTGCTCTGGGTTG(+ACTCAC)GATCCTTAGCCCCCAGGACTGGCAGTGCACA NGG 10 8 17 8 24 0 100 5 10
    240604221939_7b3x GCCACCTGCCACTGAACTCTACCAGAAAATGG(+ACTCAC)GTACCATGTCCCCTAAGAATCTAAGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240604221938_wynv AATGATGTCCACTTAGATTCTTAGGGGACATG(+ACTCAC)GTACCCATTTTCTGGTAGAGTTCAGTGGCAG NGG 10 8 17 8 24 0 100 5 10