Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222056_1tp0 AGCATGACGGTATGCATCTATCGTGAGCATAG(+ACTCAC)GATCCTAAGAGAAGGTTTAGTTAGCTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240604222054_u1fp CAGGTGTACCAGCTAACTAAACCTTCTCTTAG(+ACTCAC)GATCCTATGCTCACGATAGATGCATACCGTC NGG 10 8 17 8 24 0 100 5 10
    240604222053_azes TGCTGATGAAATCTGAACCATGCATCTTTTGG(+ACTCAC)GATCCACCTCCTTCAGTTTGGGGCAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240604222051_255e TCTGCAAGATCTGCCCCAAACTGAAGGAGGTG(+ACTCAC)GATCCCAAAAGATGCATGGTTCAGATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604222048_8dew CACTTTCCATCTTGTGTTAAACATTTACCCAG(+ACTCAC)GTACCTGGGTATTTTTGTTGTCAATTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240604222046_0cd8 TGGAAACCCCAATTGACAACAAAAATACCCAG(+ACTCAC)GTACCTGGGTAAATGTTTAACACAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240604222044_nlh7 AGCAAGGCCCTGTGTTCTTGACATCTGAGAGG(+ACTCAC)GATCCTACATATCAAGAGGGTCCGGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604222043_ezh8 AGACAAGGAGCCCGGACCCTCTTGATATGTAG(+ACTCAC)GATCCCTCTCAGATGTCAAGAACACAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240604222041_4lfk AGGTGATCCAAATGCCCTTTTGGCCCCTGCGG(+ACTCAC)GTACCACATGTATGTGGTATGTGGATCTACA NGG 10 8 17 8 24 0 100 5 10
    240604222040_ucb8 CCACATGTAGATCCACATACCACATACATGTG(+ACTCAC)GTACCCGCAGGGGCCAAAAGGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10