Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222206_qonn ACCCTGAGTAGCAGGTGGACTTGTTGGGATGG(+ACTCAC)GATCCAAGCCTGAGGGCTTACCCCATGTGGT NGG 10 8 17 8 24 0 100 5 10
    240604222204_mtpr CTGCCACCACATGGGGTAAGCCCTCAGGCTTG(+ACTCAC)GATCCCATCCCAACAAGTCCACCTGCTACTC NGG 10 8 17 8 24 0 100 5 10
    240604222203_0noh GTTAAGTTGTCTCTGTGATCCTGTGACTCTGG(+ACTCAC)GATCCTGGGACCCTGAGATCCTGGTGTGACC NGG 10 8 17 8 24 0 100 5 10
    240604222200_94b8 AGCTTGGTCACACCAGGATCTCAGGGTCCCAG(+ACTCAC)GATCCCAGAGTCACAGGATCACAGAGACAAC NGG 10 8 17 8 24 0 100 5 10
    240604222158_3214 GACCGATGAGAGAGATCCGAGTGGGTTTCCTG(+ACTCAC)GATCCCACAAAAATTTCTTCATTCTTACAAA NGG 10 8 17 8 24 0 100 5 10
    240604222156_kkm1 TTTCTTTTGTAAGAATGAAGAAATTTTTGTGG(+ACTCAC)GATCCAGGAAACCCACTCGGATCTCTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240604222154_dhjb AACCCTATCCATGCCATGTGGGGGGATGGCAG(+ACTCAC)GATCCGTCCTGAGGAATCTGTAGCCAACAAG NGG 10 8 17 8 24 0 100 5 10
    240604222153_mqe7 TAAATCTTGTTGGCTACAGATTCCTCAGGACG(+ACTCAC)GATCCTGCCATCCCCCCACATGGCATGGATA NGG 10 8 17 8 24 0 100 5 10
    240604222151_68mm TGTATGTTAATAAGCGAAACAAAATCATACGG(+ACTCAC)GATCCAGATACATTGCATCATTGTGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240604222150_s0w7 TAGAAATGGAACACAATGATGCAATGTATCTG(+ACTCAC)GATCCCGTATGATTTTGTTTCGCTTATTAAC NGG 10 8 17 8 24 0 100 5 10