Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222112_u32v GACACAGGGTGCTAAGTCTCACTGAACACTGG(+ACTCAC)GTACCCTAATAAGACTCAAACAGGCTCCATC NGG 10 8 17 8 24 0 100 5 10
    240604222110_7jhr GAGGGGATGGAGCCTGTTTGAGTCTTATTAGG(+ACTCAC)GTACCCAGTGTTCAGTGAGACTTAGCACCCT NGG 10 8 17 8 24 0 100 5 10
    240604222108_ltj8 GCTCTAGGCTGGTGGGAGAGGGGGTGTCCTGG(+ACTCAC)GATCCCCTCCAGGGACAAGTGCTCTGGGGGG NGG 10 8 17 8 24 0 100 5 10
    240604222106_g6uk AACTTCCCCCCAGAGCACTTGTCCCTGGAGGG(+ACTCAC)GATCCCAGGACACCCCCTCTCCCACCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240604222105_k1al CGTTGGATACATTGCCACAGTAACTGAAGAAG(+ACTCAC)GTACCCGGAGCCCAGTGTGAATCGTTTATAT NGG 10 8 17 8 24 0 100 5 10
    240604222103_rrt6 TTGGAATATAAACGATTCACACTGGGCTCCGG(+ACTCAC)GTACCTTCTTCAGTTACTGTGGCAATGTATC NGG 10 8 17 8 24 0 100 5 10
    240604222101_yx21 CCAGAGCCAAACCCTGTAGGTGCCCAGTGTGG(+ACTCAC)GATCCAGTATCTCATTTGGTGGTATCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240604222100_bpcw CACCAATAAAGATACCACCAAATGAGATACTG(+ACTCAC)GATCCCACACTGGGCACCTACAGGGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604222058_t3ue TTGCACTGCAAACTTGAGTTCTTTACTGAAGG(+ACTCAC)GTACCAATGCATCCATCCATAAAGGTCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604222057_dsd4 GGAGATCTGGACCTTTATGGATGGATGCATTG(+ACTCAC)GTACCCTTCAGTAAAGAACTCAAGTTTGCAG NGG 10 8 17 8 24 0 100 5 10