Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222148_nbbd ACCACATGGTGGCTCACAACCATCTGTAATGG(+ACTCAC)GATCCGATGCCCTCTTCTGGTGTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240604222144_8q3x GCTGTCTTCTGACACACCAGAAGAGGGCATCG(+ACTCAC)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240604222142_wxgw CCCATAGTAATGACCCTTAAAGTTGACCTCAG(+ACTCAC)GATCCACATACCAAGAAAAGAAATTGAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604222140_dibm CTCTAGCTCTCAATTTCTTTTCTTGGTATGTG(+ACTCAC)GATCCTGAGGTCAACTTTAAGGGTCATTACT NGG 10 8 17 8 24 0 100 5 10
    240604222139_720g ATTTTAAAATTAAGGCAGAGAGCACGAAGGAG(+ACTCAC)GTACCCAGGTCAGCCGACGGCCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240604222136_negr TGCTTGTGTGTGGAGGCCGTCGGCTGACCTGG(+ACTCAC)GTACCTCCTTCGTGCTCTCTGCCTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240604222135_hpjj TGCTCTGCCTCTCTGCTCAGGAACTAAGGATG(+ACTCAC)GTACCCTTTATCCAAAGCAGGAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240604222133_ei6a GTTTCCTCACAACTTCCTGCTTTGGATAAAGG(+ACTCAC)GTACCATCCTTAGTTCCTGAGCAGAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604222132_ha14 CAAAGGTCTAGAAAGGAAGGTTGCTTGGGCTG(+ACTCAC)GATCCCTTCCCCTCCTTTTCTCCGATTAAAT NGG 10 8 17 8 24 0 100 5 10
    240604222130_pec8 TTCGTATTTAATCGGAGAAAAGGAGGGGAAGG(+ACTCAC)GATCCAGCCCAAGCAACCTTCCTTTCTAGAC NGG 10 8 17 8 24 0 100 5 10