Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222221_y07t CTGCCTCCAGAACACTGCAGGATTCTCCACCG(+ACTCAC)GTACCTGCTGGTTTCCCATAGGCCCAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604222220_ajr9 CTGAGCAGCCTGGGCCTATGGGAAACCAGCAG(+ACTCAC)GTACCGGTGGAGAATCCTGCAGTGTTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604222219_abkh GAAGACAGAGCCCACACAGTAGGAAATTCCAG(+ACTCAC)GTACCAACACTGTAAAGTATGTGTACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240604222217_d5rz TCTCCTCTCAGTACACATACTTTACAGTGTTG(+ACTCAC)GTACCTGGAATTTCCTACTGTGTGGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240604222216_dldy TCTGTGCAGCAAAATGCTGGGTACTGTTTATG(+ACTCAC)GATCCAGTCTGTTAGTCTATGTCTCTTTATT NGG 10 8 17 8 24 0 100 5 10
    240604222215_ha50 TCCACAATAAAGAGACATAGACTAACAGACTG(+ACTCAC)GATCCATAAACAGTACCCAGCATTTTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240604222213_wqb2 AGACGAATGCCGAAGCCTGGCATCAGGGACGG(+ACTCAC)GATCCCATCCTCTGGAGTAGGCAATAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240604222211_y07j GTTTTCCACCTATTGCCTACTCCAGAGGATGG(+ACTCAC)GATCCCGTCCCTGATGCCAGGCTTCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240604222209_m4j2 AGGGGTTCCAGATGCTCAGCAGACACTCCTGG(+ACTCAC)GATCCAAGGAAGCACTTATGCCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604222207_cprw CCCCATTCCTGGTTGGCATAAGTGCTTCCTTG(+ACTCAC)GATCCCAGGAGTGTCTGCTGAGCATCTGGAA NGG 10 8 17 8 24 0 100 5 10