Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222237_veuj TCCTTGGCACCATCTCTGCTGGCACCTACTTG(+ACTCAC)GATCCCTAATACACATGAGGTGGGGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604222236_rpe4 GTTTCCCCCAACCCCACCTCATGTGTATTAGG(+ACTCAC)GATCCAAGTAGGTGCCAGCAGAGATGGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604222233_gnis ATATAATGTGGAGGCAGCAGAGGGGTAAGTGG(+ACTCAC)GTACCTAGCAGGCCCATGCCAAGGTGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240604222232_1b6j CTCAGGAGGACACCTTGGCATGGGCCTGCTAG(+ACTCAC)GTACCCACTTACCCCTCTGCTGCCTCCACAT NGG 10 8 17 8 24 0 100 5 10
    240604222231_4bw7 TGTGACCAATTATATGGTCAGTTTTGGAGAAG(+ACTCAC)GTACCATAAGGTGATGAGAAGGAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240604222229_1ffv AAAAGAATATACCTCCTTCTCATCACCTTATG(+ACTCAC)GTACCTTCTCCAAAACTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240604222228_bcvw CCCAAGCACCAGGAGAACGCAAACTAGTCTTG(+ACTCAC)GTACCAATGCTGTGAACTATAACTTGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240604222227_kx4p CTGCCCCAGCCAAGTTATAGTTCACAGCATTG(+ACTCAC)GTACCAAGACTAGTTTGCGTTCTCCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240604222225_g9im TCCCTCGATTACTCCCAGTTCAGGGAAAAGGG(+ACTCAC)GTACCAACGTGCAGACTCCTGACCAGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240604222224_mv7r TACCCGCCAGCTGGTCAGGAGTCTGCACGTTG(+ACTCAC)GTACCCCTTTTCCCTGAACTGGGAGTAATCG NGG 10 8 17 8 24 0 100 5 10