Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222343_fss1 ACAAGAAAGCCGCTCTCGTCTGCAGGACACGG(+ACTCAC)GATCCTGCGTGGCTCACAGCGGCAGCTGCCA NGG 10 8 17 8 24 0 100 5 10
    240604222342_hlx4 AGAAGTGGCAGCTGCCGCTGTGAGCCACGCAG(+ACTCAC)GATCCCGTGTCCTGCAGACGAGAGCGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604222341_mnm8 CCAGTTTTTCTCAGATTTAGGGAGAATACATG(+ACTCAC)GTACCTTTTACCTACCCATACTAGAAACTAA NGG 10 8 17 8 24 0 100 5 10
    240604222339_s3yf TGTAGTTAGTTTCTAGTATGGGTAGGTAAAAG(+ACTCAC)GTACCATGTATTCTCCCTAAATCTGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604222337_w6mz AAGTGGGGAGGAATGGTACTATTGGCATGGGG(+ACTCAC)GTACCTGGTATTGAAAATGGACACATAAACT NGG 10 8 17 8 24 0 100 5 10
    240604222335_4qd4 CAGCTAGTTTATGTGTCCATTTTCAATACCAG(+ACTCAC)GTACCCCCATGCCAATAGTACCATTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240604222334_lsjx GGGGATCTGATGTCCTCTTCTGGCCTCTGTTG(+ACTCAC)GTACCTGCACACACATGGTGTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240604222333_jizp TATTTGTGTATGTGTACACCATGTGTGTGCAG(+ACTCAC)GTACCAACAGAGGCCAGAAGAGGACATCAGA NGG 10 8 17 8 24 0 100 5 10
    240604222331_gem4 GATTCTAGTCTGACTTGGAACAGAGGTCACAG(+ACTCAC)GTACCAACCTGATGAGACCTGCTGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240604222329_imf2 GAGACACAATAGCAGCAGGTCTCATCAGGTTG(+ACTCAC)GTACCTGTGACCTCTGTTCCAAGTCAGACTA NGG 10 8 17 8 24 0 100 5 10