Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222451_ey0h GAGAATTTTCTCTCCTCCTCCACTGGGGACAG(+ACTCAC)GATCCACCAGATGTCAATAGCGTGCTTATTT NGG 10 8 17 8 24 0 100 5 10
    240604222450_z123 AGCGAAAATAAGCACGCTATTGACATCTGGTG(+ACTCAC)GATCCTGTCCCCAGTGGAGGAGGAGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240604222448_sc7b CGGCACGGCCCGCCCGGCCCCTCCACCAGGAG(+ACTCAC)GATCCGAAAGGTTACCTGTACAGTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240604222446_ci8o AGGCTACAGGAAACTGTACAGGTAACCTTTCG(+ACTCAC)GATCCTCCTGGTGGAGGGGCCGGGCGGGCCG NGG 10 8 17 8 24 0 100 5 10
    240604222445_1zxy TGGAGTCCACTGCTGGCCAGCCCCAGCACTGG(+ACTCAC)GTACCCACAGGACATGCCCCCACACACCTCA NGG 10 8 17 8 24 0 100 5 10
    240604222443_ocq6 TGTTGTGAGGTGTGTGGGGGCATGTCCTGTGG(+ACTCAC)GTACCCAGTGCTGGGGCTGGCCAGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604222441_g8bg TGTAGTCCCTCTTATCCGGCTGGATTAACCTG(+ACTCAC)GTACCCCAACATTATCCAGATTTCCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240604222439_uebz GTTTTGGAGTAGGAAATCTGGATAATGTTGGG(+ACTCAC)GTACCAGGTTAATCCAGCCGGATAAGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240604222438_b0ja CATCTCTGGAACTCGGATTCCCATCGTATTGG(+ACTCAC)GTACCACTGCTTTTAAAGCACTAAGTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240604222436_4wfu TATGTTTAACACTTAGTGCTTTAAAAGCAGTG(+ACTCAC)GTACCCAATACGATGGGAATCCGAGTTCCAG NGG 10 8 17 8 24 0 100 5 10