Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223250_undm GGTTTATGATGAAAACTTTATTCTGCATCAGG(+ACTCAC)GTACCTGGAAAGTGAAGCTGCATTTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240604223248_4d5m CCTGCATTACCTGCCAGCGAGTCAAGGTAGGG(+ACTCAC)GATCCAGCAAACGGTTTTGGGAATTCAGACT NGG 10 8 17 8 24 0 100 5 10
    240604223246_6mmi TCCACAGTCTGAATTCCCAAAACCGTTTGCTG(+ACTCAC)GATCCCCTACCTTGACTCGCTGGCAGGTAAT NGG 10 8 17 8 24 0 100 5 10
    240604223244_ofbd TGAAGCACTCTGCATCCTCAGGGGAACTTGGG(+ACTCAC)GTACCCAATGGAAACATTTGTAAAAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240604223243_4uxo ATTTAATGAACTTTTACAAATGTTTCCATTGG(+ACTCAC)GTACCCCAAGTTCCCCTGAGGATGCAGAGTG NGG 10 8 17 8 24 0 100 5 10
    240604223241_phwu GCTGTCTTCAGACACACCAGAAGAGGGCATTG(+ACTCAC)GATCCCATTACAGATGGTTGTAAACCACTAT NGG 10 8 17 8 24 0 100 5 10
    240604223239_4fvq ACCACATAGTGGTTTACAACCATCTGTAATGG(+ACTCAC)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604223238_uojl GGTGGAATTATGATGGAGTTCTGACTTTATTG(+ACTCAC)GTACCATGAAAGTCAGGGTATACTTGACTAA NGG 10 8 17 8 24 0 100 5 10
    240604223236_ymrc ATACTTTAGTCAAGTATACCCTGACTTTCATG(+ACTCAC)GTACCAATAAAGTCAGAACTCCATCATAATT NGG 10 8 17 8 24 0 100 5 10
    240604223235_bc1m CAGCCTTCCAAGTCATGAACCTTCTTTGAGAG(+ACTCAC)GATCCACCCCACTAGTCCTTCCCCCTCGCAC NGG 10 8 17 8 24 0 100 5 10