Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222400_vfmm CCGAGTCCAGGTGCCCATGGAAGAAAGCATTG(+ACTCAC)GATCCCCCTGGAGTTGAAGTCACCTAATGTA NGG 10 8 17 8 24 0 100 5 10
    240604222358_dvud ACATCTACATTAGGTGACTTCAACTCCAGGGG(+ACTCAC)GATCCAATGCTTTCTTCCATGGGCACCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604222356_aqxo AAAAGACCCCAGAAGCACGTGGGTGAGGCCTG(+ACTCAC)GATCCAGGCACCTGTGGGAGGTGAGCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604222354_v44x ACATGAGGAGGCTCACCTCCCACAGGTGCCTG(+ACTCAC)GATCCAGGCCTCACCCACGTGCTTCTGGGGT NGG 10 8 17 8 24 0 100 5 10
    240604222352_2rvi TGGTCAAGCTCAGCAGCCCCTGGGTCCCCTTG(+ACTCAC)GTACCAGTCAAAGGTGGAGCAATCCCGAACT NGG 10 8 17 8 24 0 100 5 10
    240604222351_4yde CCCAAAGTTCGGGATTGCTCCACCTTTGACTG(+ACTCAC)GTACCAAGGGGACCCAGGGGCTGCTGAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604222349_oqar GGCCACCCCAACAGATGGGCTCCCTTCTATGG(+ACTCAC)GATCCCCAAAGGGCAGGTCCTGGCCGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240604222348_9ono AGCTGTCCAGCGGCCAGGACCTGCCCTTTGGG(+ACTCAC)GATCCCATAGAAGGGAGCCCATCTGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240604222346_1hc0 ATGCCATGGCTGCACTGGACACAGGAGAAGGG(+ACTCAC)GTACCAGTCACTGACGCCAGGCTGCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604222344_22m9 AGGGTGGCACAGCAGCCTGGCGTCAGTGACTG(+ACTCAC)GTACCCCTTCTCCTGTGTCCAGTGCAGCCAT NGG 10 8 17 8 24 0 100 5 10