Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222418_i2g9 CACGATGCCAGGTCTGGGGCACACTGTGGTTG(+ACTCAC)GATCCAGCTGGTCACATCCAGATGGGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240604222417_xbyj ACCCCACTCCCCCATCTGGATGTGACCAGCTG(+ACTCAC)GATCCAACCACAGTGTGCCCCAGACCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240604222414_xj63 TTATGATCCCGGTGCTGAGGAGGTGGTGGCGG(+ACTCAC)GATCCCTGGAGCTCACTGGCAAGCCTCATCA NGG 10 8 17 8 24 0 100 5 10
    240604222412_z9gx CTCCCTGATGAGGCTTGCCAGTGAGCTCCAGG(+ACTCAC)GATCCCGCCACCACCTCCTCAGCACCGGGAT NGG 10 8 17 8 24 0 100 5 10
    240604222411_gfky TGTGGCCCTCCTGGAGCAACCTGGCAGCTACG(+ACTCAC)GATCCCAGAACCCCCTGGGCTTCAGCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240604222409_5vuc CTCTGGGGGAGCTGAAGCCCAGGGGGTTCTGG(+ACTCAC)GATCCGTAGCTGCCAGGTTGCTCCAGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240604222407_q6u4 CAGCACGAGGCTCGCCGTGAAGGGTGGCCTCG(+ACTCAC)GATCCTGGGCACGGCACGGTGACACTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240604222405_fpuw CATCTGTTGCAGTGTCACCGTGCCGTGCCCAG(+ACTCAC)GATCCGAGGCCACCCTTCACGGCGAGCCTCG NGG 10 8 17 8 24 0 100 5 10
    240604222403_k0t9 TAGGGGCGCAGGGCGCGTGGAACCTAGCTGGG(+ACTCAC)GATCCAGCTTGCCTGGGTTTCTGGGGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240604222401_tyn6 CTGTTTACCACCCCAGAAACCCAGGCAAGCTG(+ACTCAC)GATCCCCAGCTAGGTTCCACGCGCCCTGCGC NGG 10 8 17 8 24 0 100 5 10